Fig. 5: 8-oxo-rGTP(syn) insertion opposite adenine.
From: Structural basis for proficient oxidized ribonucleotide insertion in double strand break repair
a Active site of the Mn2+-reaction state (RS) ternary complex of pol μ in the process of inserting 8-oxo-rGTP(syn) opposite At. Arrows indicate bond broken (substrate) and formed (product). Protein sidechains are shown in yellow stick representation, the incoming nucleotide is green and DNA is cyan. Mn2+ atoms are shown as magenta spheres. Simulated annealing omit density is shown as a green mesh contoured at 3 σ. b Active site of the 8-oxo-rGMP(syn):At Mn2+-product (PS) complex. A red arrow indicates rotation of Asp330 compared to the ground and reaction states. Water molecules are shown as blue spheres. c Post-catalytic (960 min) Mg2+:8-oxo-rGMP(syn):At product (ES) complex. The larger purple sphere is Na+, while Mg2+ is a green sphere.
