Fig. 1: Loss of Nf1 increases CO2 production via neuronal mechanisms.
From: Neurofibromin regulates metabolic rate via neuronal mechanisms in Drosophila
a Diagram of respirometry apparatus. b Normalized CO2 production in Nf1P1 mutants and wCS10 controls. **p = 0.002 (Mann–Whitney, two-sided; n = 9). c Normalized CO2 production in heteroallelic Nf1P1/E1 mutants (n = 12) and controls (n = 11). ***p < <0.0001 (Mann–Whitney, two-sided). d Normalized CO2 production when Nf1 was knocked down with RNAi driven by pan-neuronal Gal4 lines: nSyb- and R57C10-Gal4. Gal4/+ and UAS/+ are heterozygous controls. ***p < 0.001 (Dunn’s test, two-sided; for nSyb-Gal4: n = 8 UAS-Nf1 RNAi/+, n = 10 nSyb-Gal4/+, n = 11 experimental group. For R57C10-Gal4: n = 12 per genotype). Each “n” = single respirometer (containing 4 animals). Box plots—box: 1st to 3rd quartiles, median: line, whiskers: min–max, individual data points: circles.
