Fig. 5: Insulin-mediated protection against palmitoleic-induced Ca²⁺ overload is abolished in pancreatic acinar cells from PACIRKO mice.

Representative traces showing POA-induced [Ca²⁺]_i responses (a–d) in untreated fura-2-loaded pancreatic acinar cells (a, c) and following pre-treatment with 10 nM insulin for 15 min (b, d) from IR^lox/lox (a, b) and PACIRKO (c, d). Cells were also subsequently treated with 30 pM CCK to test for recoverability and thus cell viability post-POA treatment. Mean (±SEM) maximum increase in resting [Ca²⁺]_i above baseline (e) and mean (±SEM) area under the curve (AUC; f) over the treatment and recovery period in the absence (dark grey bars) or following treatment with 10 nM insulin (light grey bars). Significance (specific p values as indicated) was determined by one-way ANOVA with Sidak’s multiple comparisons. Data were derived from individual values from multiple cells (6-36 cells per experiment) in the field of view for each experiment. These values were averaged giving the experimental mean, that were in turn averaged across multiple experiments (four separate experiments for each experimental condition, except PACIRKO with POA, n = 5) giving the true mean ± SEM as indicated in (e, f).