Fig. 2: Genetic mapping of the GTRC locus.

a Mapping the gtrC-5 mutant. Left, segregants from a gtrC-5/Vx hybrid spore capsule. Right: The genetic interval containing the gtrC-5 locus was identified to a 840 kb region (GoldenGate: between 61.605 and 64.69 cM,) in the first round of mapping, and reduced to the interval of 283 kb distance in the second round of manual genotyping. A candidate gene with a nonsense mutation was identified in this reduced interval. Source data are provided as a Source Data file. b Mapping the gtrC-16 mutants. Left, segregants from gtrC-16/Vx hybrids. Right, Gd/Vx SNP ratios in chromosome 2 derived from 48 gtrC-16/Vx hybrid progeny whose protonemata grew downwards. They were mixed and DNA was extracted for whole-genome sequence. Red lines are plots of mean values of Gd/Vx SNP ratios in a 0.5 Mb region with the sliding window set to 0.25 Mb. A candidate gene with nonsense mutation was identified. Source data are provided as a Source Data file. In a, b, the arrows with “g” show the direction of the gravity vector. The segregants were divided into three groups based on the growth direction of dark-grown protonemata: Up, Down, or Undetermined (Undet.). Scale bars, 10 mm. The numbers (No.) of each phenotype were shown in the tables below each image. c Gene structure and mutation sites of the GTRC locus. Purple box, untranslated region; orange box, coding exons. X indicates stop codon. d Protein structure and the mutation sites of GTRC. X indicates stop codon. Brown box, CH domain; green box, coiled coil region predicted by COILS program; red box, motor domain; blue box, C terminus of KCH proteins.