Fig. 2: Atg8, p62, and ubiquitinated nuclear proteins accumulate in cells lacking TER94 function. | Nature Communications

Fig. 2: Atg8, p62, and ubiquitinated nuclear proteins accumulate in cells lacking TER94 function.

From: VCP maintains nuclear size by regulating the DNA damage-associated MDC1–p53–autophagy axis in Drosophila

Fig. 2: Atg8, p62, and ubiquitinated nuclear proteins accumulate in cells lacking TER94 function.The alternative text for this image may have been generated using AI.

a Time-course analysis of the nuclear size change and the presence of ubiquitinated proteins in Rh1 > LacZ and Rh1 > TER94K2A pupal or adult eyes stained with phalloidin (red), anti-Lamin (magenta), and anti-Ubiquitin conjugates (FK2, green) antibodies. b Pearson’s correlation analysis of nuclear cross-section size and FK2 intensity from images of 1-day-old adult retina (as shown in panel a). The number of independent nuclei measured are 88 (LacZ) and 125 (TER94K2A). r values represent Pearson’s correlation coefficient in each group. c An adult TER94K15502 clone, marked by the absence of myr.GFP (arrowhead), stained with anti-Lamin (magenta) and anti-Ubiquitin conjugates (FK2, red) antibodies. R cell boundaries are outlined in dash lines. d, e Retinas from freshly eclosed (0-day) to 5-day-old adults co-expressing GFP-Atg8a (d) or GFP-p62 (e) with LacZ (control) or TER94K2A under the control of Rh1-GAL4 stained with phalloidin (red) and anti-Lamin (magenta) antibody. The number of independent experiments performed: a 2 (LacZ), 5 (TER94K2A) for 90% pupal; 3 (LacZ), 2 (TER94K2A) for 0-day; 4 (LacZ), 6 (TER94K2A) for 1-day; 6 (LacZ), 6 (TER94K2A) for 5-day adult. c 6. d 3 (0-day), 2 (1-day), 6 (2-day), 3 (5-day). e 2 (0-day), 5 (1-day), 5 (2-day), 4 (5-day). Scale bars: 10 µm (a, d, e), 5 µm (c).

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