Fig. 4: Ex vivo IFNγ ELISpot responses in individuals at baseline and 28–32 days following a single dose of the AZD1222/Covishield vaccine.
From: Immune responses to a single dose of the AZD1222/Covishield vaccine in health care workers
Ex vivo IFNγ ELISpot responses were measured to two pools representing the spike protein (S1 and S2) at the baseline (pre) and 28–32 days following the vaccine in total naïve individuals (n = 72) (A), and in different age sub-groups of naïve individuals (n = 68) (B). The association of the ex vivo IFNγ ELISpot responses to the two pools of the spike protein (S1 and S2) and the total responses to overlapping peptides of the spike protein did not correlate with the total antibody responses (n = 68) (C). The Wilcoxon matched-pairs signed-rank test was used to compare the means of ex vivo ELISpot responses to S1 and S2 pool of peptides before and after the vaccine. The Kruskal–Wallis test was used to compare the S1 and S2 ELISpot responses in different age groups. Spearman rank order correlation coefficient was used to evaluate the correlation between the ex vivo ELISpot responses and the SARS-CoV-2-specific antibodies (antibody index). All tests were two sided. Data are presented as median values ± interquartile ranges as appropriate.
