Fig. 5: Paternally biased expression of INS-IGF2 transcript. | Nature Communications

Fig. 5: Paternally biased expression of INS-IGF2 transcript.

From: Large parental differences in chromatin organization in pancreatic beta cell line explaining diabetes susceptibility effects

Fig. 5

a Gene isoforms at IGF2-INS locus. Data is retrieved from UCSC Genome Browser42 hg19 assembly from chr11:2150251-2182750. Approximate position of SNP rs3168310 is marked with a star in the last exon of the IGF2 gene, and that of SNP rs689 with a diamond in the first intron of the INS gene. Isoform 1 of INS-IGF2 transcript is marked with an arrow. b Sequencing INS-IGF2 isoform 1 PCR product shows that at SNP rs3168310, the G allele is the major product over C allele (71.9% G, 28.1% C). Sequencing primer reads from the reverse strand. c–f SNP typing H19-ICR-IGF2 3C product. The IGF2 rs3168310 G allele (c) is found in the same PCR product with the H19-ICR rs113013264 A allele (d). Meanwhile, the IGF2 rs3168310 C allele (e) is found in the same PCR product with the H19-ICR rs113013264 G allele (f). g Genotyping of rs689. Sanger sequencing showed that in EndoC-βH1 cells, SNP rs689 is the monoallelic A allele. The sequencing primer reads from the forward strand.

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