Fig. 2: Intracellular Ca²⁺ signals in single FDB muscle cells isolated from WT and R2509C mice.

a Structure of Cpd1 sodium salt. b Representative Ca²⁺ signals in the isolated FDB muscle cells from WT and R2509C mice at various concentrations of halothane in the absence (WT and R2509C) and presence of 0.1 μM Cpd1 (R2509C, Cpd1). Fluorescence Ca²⁺ signals (F) were detected with Cal520 and normalized to the initial value (F₀). c Average resting Ca²⁺ signals at various concentrations of halothane and effects of 0.1 μM Cpd1. Data are shown as means ± SD (WT: n = 4, R2509C: n = 7, R2509C, Cpd1: n = 5) and were analyzed by one-way ANOVA with Tukey’s test. d Average Ca²⁺ signals obtained with fura-2 at rest and in the presence of Cpd1. Data are shown as box-whisker plots, with the median for all subjects shown as the center line, the box representing the 25–75 percentile, and the lines showing the range of the data (WT, 0 µM: n = 34; WT, 0.1 µM: n = 21; WT, 0.3 µM: n = 16; R2509C, 0 µM: n = 45; R2509C, 0.1 µM: n = 28; R2509C, 0.3 µM: n = 17) and were analyzed by two-way ANOVA with Tukey’s test. e Representative fura-2 ratio signals in isolated FDB muscle cells from WT and R2509C mice at various concentrations of isoflurane in the absence (WT and R2509C) and presence of 0.1 μM Cpd1 (R2509C, Cpd1). f Average resting Ca²⁺ signals at various concentrations of isoflurane and effects of 0.1 μM Cpd1. Data are shown as means ± SD (WT: n = 11; R2509C: n = 8 or 10, 2 cells died after exposure to 0.5% isoflurane; R2509C, Cpd1: n = 8) and were analyzed by one-way ANOVA with Tukey’s test. Source data are provided as a Source Data file.