Fig. 4: In vivo isoflurane challenge in WT and R2509C mice. | Nature Communications

Fig. 4: In vivo isoflurane challenge in WT and R2509C mice.

From: A novel RyR1-selective inhibitor prevents and rescues sudden death in mouse models of malignant hyperthermia and heat stroke

Fig. 4

a–c Preventive effects of Cpd1. a Time course of rectal temperature in mice after anesthesia by isoflurane. Cpd1 (0, 3, 10 mg/kg) was administered i.p. to R2509C mice 10 min before anesthesia. †, death by fulminant MH crisis. b Maximum rectal temperature. Data are shown as means ± SD (WT: n = 6; R2509C, 0 mg/kg: n = 9; R2509C, 3 mg/kg: n = 6; R2509C, 10 mg/kg: n = 6) and were analyzed by one-way ANOVA with Tukey’s test. c Survival rate of mice after 90 min of anesthesia. Administration of Cpd1 at 10 mg/kg but not 3 mg/kg prevented R2509C mice from fulminant MH crisis. d–f Rescue effects of Cpd1 on fulminant MH crisis in R2509C mice. d Time course of rectal temperature in mice after administration of Cpd1 during anesthesia. Mice were anesthetized by isoflurane and Cpd1 (0, 3, 10 mg/kg) was administered i.p. when their body temperature reached 39 °C (arrow). †, death by fulminant MH crisis. e Change in rectal temperature of R2509C mice from 39 °C at 10 min after the administration of Cpd1 or saline control and the endpoint (60 min after administration or just before death). Data are shown as means ± SD (0 mg/kg: n = 16; 3 mg/kg: n = 10; 10 mg/kg: n = 8) and were analyzed by one-way ANOVA with Tukey’s test. f Survival rate of R2509C mice 60 min after administration of Cpd1 or saline control. Source data are provided as a Source Data file.

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