Fig. 5: In vivo heat stress challenge in WT and R2509C mice. | Nature Communications

Fig. 5: In vivo heat stress challenge in WT and R2509C mice.

From: A novel RyR1-selective inhibitor prevents and rescues sudden death in mouse models of malignant hyperthermia and heat stroke

Fig. 5

Anesthetized mice were placed in a test chamber prewarmed at 35 °C. a–c Cpd1 (10 mg/kg) was administered 10 min before heat stress challenge. a Time course of the change of rectal temperature of mice. b Maximum rectal temperature. Data are shown as means ± SD (WT: n = 7; R2509C, 0 mg/kg: n = 13; R2509C, 10 mg/kg: n = 4) and were analyzed by one-way ANOVA with Tukey’s test. c Survival rate of mice 120 min after heat stress challenge. d Time to death from start of heat stress challenge. Data are shown as means ± SD (0 mg/kg: n = 13; 10 mg/kg: n = 4) and were analyzed by two-tailed unpaired t-test. e–g Rescue effects of Cpd1 on heat stroke in R2509C mice. e Time course of rectal temperature of mice. Cpd1 (0, 3, 10 mg/kg) was administered i.p. when their body temperature reached 39 °C (arrow). †, death by heat stroke. f Change in the rectal temperature from 39 °C at 10 min after the administration of Cpd1 or saline control and the endpoint (60 min after administration or just before death). Data are shown as means ± SD (0 mg/kg: n = 12; 3 mg/kg: n = 5; 10 mg/kg: n = 11) and were analyzed by one-way ANOVA with Tukey’s test. g Survival rate of R2509C mice 60 min after administration of Cpd1 or saline control. Source data are provided as a Source Data file.

Back to article page