Fig. 1: The construction procedure of the genome-reduced mutants of DSM 7029. | Nature Communications

Fig. 1: The construction procedure of the genome-reduced mutants of DSM 7029.

From: Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria

Fig. 1: The construction procedure of the genome-reduced mutants of DSM 7029.

Circular genome map of the DSM 7029 wild type (WT), targeted deletion regions of biosynthetic gene clusters (BGCs) were marked with bold stubs of different colors and targeted deletion regions of transposases, GIs, prophages were marked with two opposite triangles of different colors on the physical localizations of the chromosome. The route 1 represents the sequential deletions of eight endogenous BGCs, resulting in the seven genome-reduced DSM 7029 strains DC1–DC7. The route 2 represents sequential removals of several transposases, GIs, prophages, and vicinal nonessential genes, leading to the ten genome-reduced DSM 7029 strains DT1–DT10.

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