Fig. 6: Discovery of chitinimides by heterologous expression of chm BGC in genome-reduced chassis. | Nature Communications

Fig. 6: Discovery of chitinimides by heterologous expression of chm BGC in genome-reduced chassis.

From: Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria

Fig. 6: Discovery of chitinimides by heterologous expression of chm BGC in genome-reduced chassis.

a Construct of p15A-phiC31-Papra-chm with p15A replicon (blue), oriT-attP-phiC31 cassette (gray), and chm BGC (red) was under the control of Papra constitutive promoter (gray arrow upstream of the chmA). b Yield comparison of chitinimides (A and B) in E. coli GB05-MtaA (GB05), P. putida KT2440 (KT2440), native producer C. koreensis DSM 17726 (DSM 17726), DSM 7029 wild type (WT) and DT6–DT10 mutants. Data are presented as mean values ± SD. n = 3 biologically independent samples. P values were determined by two-tailed unpaired t test. *p < 0.05; ** p < 0.01; *** p < 0.001. Source data and the exact p values are provided as a Source Data file. c The bioinformatics analysis of chm BGC from C. koreensis DSM 17726. The chm BGC (top) shares many ORFs that of high similarity with the detoxin and rimosamide BGCs in Streptomyces spp. and an undefined BGC in Pseudomonas sp. Homologous ORFs are marked with identical color. d The structures of chitinimide A-H (1–8), and the structures of detoxin and rhimosamides.

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