Fig. 6: SREBF1, TP63, and KLF5 cooperatively regulate SCC cell transcriptome.

a Heatmaps of ChIP-Seq signals at SREBF1 peak regions (±3 Kb of peak center), grouped as ubiquitous, shared or cell-type-specific peak sets, and rank ordered by intensity of SREBF1 peaks based on reads per million mapped reads (RPM). Lines, peaks; color scale of peak intensity is shown at the bottom. b Representative top shared or cell-type-specific TF motifs (denoted by *) in each peak sets. Note that KLF5 motif was found in both ESCC and HepG2 promoter regions (denoted by **). P-values were adjusted for multiple comparisons. c IGV tracks of H3K27Ac and SREBF1 ChIP-Seq profiles at the loci of representative genes from each peak set. d Heatmaps of ChIP-Seq signals at ESCC-specific SREBF1 peaks grouped as either trio-, dual-, or solo-occupied by SREBF1/TP63/KLF5 (±5 Kb of peak center), rank ordered by intensity of SREBF1 peaks. e Similar as d, line plots showing the distribution of indicated ChIP-Seq signals at ESCC-specific SREBF1 peak regions from indicated groups. f GSEA plots of the changes of the corresponding transcripts assigned to the 274 ESCC-specific peaks from RNA-Seq upon silencing of either SREBF1, TP63, or KLF5 in TE5 cells. NES normalized enrichment score. P-values were adjusted for multiple comparisons.