Fig. 7: SREBF1, TP63, and KLF5 cooperate to transcriptionally activate ErbB/mTOR signaling pathways in ESCC cells. | Nature Communications

Fig. 7: SREBF1, TP63, and KLF5 cooperate to transcriptionally activate ErbB/mTOR signaling pathways in ESCC cells.

From: Interplay and cooperation between SREBF1 and master transcription factors regulate lipid metabolism and tumor-promoting pathways in squamous cancer

Fig. 7

a Significantly enriched KEGG pathways of the corresponding transcripts assigned to the ubiquitous peaks or b the 274 ESCC-specific peaks trio-occupied by SREBF1, TP63, and KLF5. The number of genes enriched/the total number of genes in each pathway are shown in the brackets. c Left panel showing genes enriched in ErbB/mTOR pathways, which were occupied by SREBF1/TP63/KLF5 based on ChIP-Seq data. Black, occupied. Right panel: the fold changes (knockdown v.s. scramble control) of mRNA levels of indicated genes upon the silencing of either SREBF1, TP63, or KLF5 in three cell lines. In each group, 30 nM siRNA was transfected; after 48 h, total RNA was extracted for the measurement the mRNA expression by qRT-PCR. d IGV tracks of ChIP-Seq profiles of indicated factors at WNT9A gene locus in different cell lines. e Western blotting analyses following siRNA knockdown of SREBF1 in ESCC cells. Quantification of the blots is shown below. f A model diagram of the regulations and functions of SREBF1 in SCC cells.

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