Fig. 4: MS identification of S11IP N-terminal Ub peptide. | Nature Communications

Fig. 4: MS identification of S11IP N-terminal Ub peptide.

From: Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W

Fig. 4

a Extracted ion chromatograms (±4 ppm) for N-terminal GGX peptide GGMFGSAPQRPVAMTTAQR in anti-K-ε-GG, anti-GGX 2B12, and anti-GGX 1C7 immunoaffinity enrichment MS experiments. b MS/MS spectrum identification of triply charged 654.9938 m/z N-terminal GGX modified peptide GGMFGSAPQRPVAMTTAQR. Detected b- and y- ions highlighted in blue and red, respectively.

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