Table 1 UCHL1 and UCHL5 kinetics table for Ub-Rho110-monoubiquitin experiment.
From: Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
Protein | Km (μM) | kcat (s−1) | kcat/Km (s−1 μM−1) |
|---|---|---|---|
UCHL1 | 0.2009 | 0.093 | 0.4 |
UbG76V-UCHL1 | 0.13 | 0.000267 | 0.002 |
UbI44A,G76V-UCHL1 | 0.198 | 0.005356 | 0.027 |
UCHL1C90S | ND | ND | ND |
UCHL5 | 12.38 | 2.792 | 0.22 |
UbG76V-UCHL5 | 6.395 | 6.112 | 0.95 |
UbI44A,G76V-UCHL5 | 8.875 | 6.268 | 0.70 |
UCHL5C88S | ND | ND | ND |
