Fig. 1: ScRNA-seq characterization of ATMs. | Nature Communications

Fig. 1: ScRNA-seq characterization of ATMs.

From: Role of Tim4 in the regulation of ABCA1+ adipose tissue macrophages and post-prandial cholesterol levels

Fig. 1

a CD45+Lin−CD11b+F4/80+ ATMs from the epididymal AT (EAT) of mice kept on CD (n = 3) or fed a HFD overnight (n = 3) were cell-sorted and underwent scRNA-seq. Lineage (Lin) includes TCRb, CD19, SiglecF, and Ly6G. b Unsupervised clustering of ATMs with UMAP where each dot is a single cell colored by cluster assignment. c Repartition of ATMs in each cluster per condition. d Heatmap of each cell’s (column) scaled expression of the top 25 conserved DEGs (row) expressed per cluster, with exemplar genes labeled (right). e Violin plots of canonical ATM gene expression by cluster. f–h Slingshot analysis of ATM trajectory in mice kept on control diet. UMAP visualization of the pseudotime values with Cluster 5 as starting point (f, g). Heat map with spline curves fitted to DEGs along a trajectory from ATMs in Cluster 5 to ATMs in Cluster 1 (h).

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