Fig. 1: Establishment of the PIC expression profiling method.
From: High-depth spatial transcriptome analysis by photo-isolation chemistry
a Schematic overview of expression profiling with PIC. PT7, T7 promoter; T7 Pol, T7 polymerase. b Strategy (top) and the result (bottom) of primer extension experiments to suppress read-through of DNA polymerase I at the NPOM-caged dT sites (black circles) without UV irradiation. A representative image was shown out of three independent experiments. c Various caged ODNs were examined for RNA-seq library preparation with (circles) or without (crosses) photo-irradiation before quantifying the expression of Actb, Gapdh, Gusb, and Eef1a genes. d Sequence of the caged ODN showing the lowest background in (c). e Effect of HCl permeabilisation before in situ RT in GFP-expressing NIH/3T3 cells was evaluated by the yield of Gfp cDNA (left) and the size (right) of sequence libraries. A representative image was shown out of three independent experiments. f GFP-expressing NIH/3T3 cells were photo-irradiated for various times after in situ RT, and the yield of Gfp cDNA was quantified. Source Data is available as a Source Data file.
