Fig. 2: Validation of PIC for ROI-specific profiling.
From: High-depth spatial transcriptome analysis by photo-isolation chemistry
a–e Experiments to evaluate the level of background from nonirradiated cells were performed with human–mouse mixed cultures (a, b) and E14.5 mouse embryos (c–e). After photo-irradiation (pink) of either human (T-47D, grey) or mouse (GFP-expressing NIH3T3, green) cells (a), the cDNAs of human (GAPDH) and mouse (Gapdh and Gfp) genes were examined (b). Mouse embryonic sections of Sox2-positive (neural tube, green) or -negative (hindlimb) cells (c, d a representative image was shown out of the replicates) were photo-irradiated and the cDNAs of Sox2 and Gapdh genes were examined (e). Scale bars, 50 μm; red in D, nuclei. Source Data is available as a Source Data file.
