Fig. 1: Identification of Lsm12 as a putative NAADP receptor. | Nature Communications

Fig. 1: Identification of Lsm12 as a putative NAADP receptor.

From: Lsm12 is an NAADP receptor and a two-pore channel regulatory protein required for calcium mobilization from acidic organelles

Fig. 1

a Strategies of affinity precipitation- and SILAC-based quantitative proteomic analyses of interacting proteins of NAADP (left) and TPCs (right). b Chemical structures of immobilized NAADP. c Numbers of overlapping and non-overlapping putative interacting proteins identified by MS in the indicated four types of affinity-precipitated samples. d Representative heavy-light peak pairs (black) of Lsm12 peptides. Peptide SQAQQPQKEAALS194 (top) was identified in the samples affinity-purified by immobilized NAADP from TPC1- and TPC2-expressing cells. Peptide LQGEVVAFDYQSK37 was identified in the TPC1 and TPC2 complexes affinity-purified by an anti-FLAG antibody. e Immunoblot of endogenous Lsm12 in samples immunoprecipitated by an anti-FLAG antibody from cells expressing FLAG-tagged TPC1 or TPC2. f Immunoblot of endogenous Lsm12 in samples pulled down by immobilized NAADP from TPC1- and TPC2-expressing cells. AP affinity-precipitation. Exp. transient expression, IB immunoblot.

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