Fig. 3: Lsm12 functions as a high-affinity NAADP receptor.

a Plot of the relationship between hLsm12-HisE.coli pulled down by immobilized NAADP and the concentrations of free NAADP and NADP. b Competition radioligand binding assay of the association between 32P-NAADP and purified hLsm12-HisE.coli in the absence or presence of various concentrations of non-radiolabeled NAADP and NADP. c Plot of the relationship between HEK293 endogenous Lsm12 pulled down by immobilized NAADP and the concentrations of free NAADP and NADP. Data were presented as mean value ± SEM.