Fig. 7: Lsm12 function in NAADP signaling is compromised by Δ45–50 mutation in MEFs and HEK293 cells.

a Averaged NAADP-induced changes in Ca²⁺ indicator fluorescence in MEFs prepared from WT and Lsm12^Δ45-50 mutant mice. b Averaged NAADP-induced changes in Ca²⁺ indicator fluorescence in HEK293 Lsm12-KO cells transiently expressing TPC2 and Lsm12 WT or Δ45–50 mutant. c Averaged current density of NAADP (100 nM in pipette solution) microinjection-induced whole-cell currents in HEK293 Lsm12-KO cells transiently expressing TPC2^PM and Lsm12 WT or Δ45–50 mutant. d Immunoblot of Lsm12 in MEFs prepared from WT and Lsm12^Δ45–50 mutant mice. MEFs were prepared and pooled from five WT and six Lsm12^Δ45–50 mutant mice, respectively. e Co-immunoprecipitation (IP) of TPC2 (V5-tagged) and LSM12 WT or Δ45–50 mutant transiently expressed in HEK293 LSM12-KO cells. f Immunoblot of Lsm12 WT and Δ45–50 mutant that was transiently expressed in HEK293 Lsm12-KO cells and pulled down by immobilized NAADP in the presence of different concentrations of free NAADP. Data were presented as mean value ± SEM. Unpaired Student’s t-test (two-tailed) was used to calculate p values. *, ***, and ***** are for p values ≤ 0.05, 0.001, and 0.00001, respectively. IB immunoblot, IP immunoprecipitation.