Fig. 4: Transcriptional profiling of mosaic RNF20/40-depleted CMs. | Nature Communications

Fig. 4: Transcriptional profiling of mosaic RNF20/40-depleted CMs.

From: Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation

Fig. 4

a Experiment overview. b Principal component analysis of RNA-seq replicates. c Differentially expressed genes. Red indicates Benjamini–Hochberg-corrected Wald test P < 0.05. d, e Gene set enrichment analysis with custom gene sets for “Adult Specific Genes” (d) or “Neonatal Specific Genes” (e). RNF20/40-depleted cells were depleted for adult-specific genes and enriched for adult-specific genes. f Gene set enrichment analysis of indicated KEGG pathway gene sets, using genes ranked by their expression in RNF20/40-depleted CMs compared with control CMs. GSEA nominal P-val is not corrected for set size or multiple-hypothesis testing, the Normalized Enrichment Score. Source data are provided as a Source Data file.

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