Fig. 2: Tfam is critical for γδT17 cell and ILC3 maintenance. | Nature Communications

Fig. 2: Tfam is critical for γδT17 cell and ILC3 maintenance.

From: Mitochondrial transcription factor A in RORγt+ lymphocytes regulate small intestine homeostasis and metabolism

Fig. 2

a Flow cytometry analyses of ILC3s (upper panel, gated on lineage-negative lymphocytes) and γδT17 cells (lower panel, gated on γδT cells) from small intestine lamina propria (LPL) of 3-week-old mice of indicated genotypes. Representative data of three independent experiments. Lineage markers in b and c include CD3, CD5, CD19, Ly6G, CD11b, and CD11c. b γδT17 cell percentages in total γδT cells (percentage, *P = 0.0120; cell number, *P = 0.0106), and ILC3 percentages in lineage-negative (Lin–) cells (upper panel) (P = 0.1575) and cell numbers (lower panel) (P = 0.1725) from the small intestine of mice shown in a (n = 4 for each group). Compiled data from two independent experiments. c γδT17 cell percentages in total γδT cells and ILC3 percentage in lineage-negative cells (upper panel) (γδT17: week 3, ***P = 0.0002, week 6, ****P < 0.0001; ILC3: week 6, **P = 0.0019) and cell numbers (lower panel) (γδT17: week 3, **P = 0.0035, week 6, *P = 0.0101; ILC3: week 6, *P = 0.0396) of the large intestine of mice at indicated ages (n = 4 for each group). The rest time points showed no significance between Ctr and Tfamfl/flRorc-cre (P > 0.05). Compiled data from two independent experiments. d Th17 cell percentages in total CD4+ T cells (upper panel) (P = 0.1705) and cell numbers (lower panel) (P = 0.0950) of the large intestine of 6-week-old age mice (n = 4 for each group). Compiled data from two independent experiments. e RORγt expression in γδT cells of various organs (Sp spleen, pLN peripheral lymph nodes) of mice with indicated genotypes measured by flow cytometry. Representative data of three independent experiments. f γδT17 cell percentages in total γδT cells from the various organs as shown in e (n = 4 for each group). Sp, *P = 0.0121; pLN, ****P < 0.0001; liver, **P = 0.0043; lung, ***P = 0.0009; fat, ****P < 0.0001; skin, ****P < 0.0001. Compiled data from two independent experiments. Ctr included Tfam+/+, Tfamfl/+, Tfamfl/fl, Tfam+/+Rorc-cre, and Tfamfl/+Rorc-cre mice. Data are shown as mean ± SD in b–d, f.

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