Fig. 6: Vascular colonization of arterioles leads to limited neutrophil recruitment and efficiency.
From: Colonization of dermal arterioles by Neisseria meningitidis provides a safe haven from neutrophils
a Intravital imaging (maximum intensity z-projection) of intraluminal (top panel) or perivascular (bottom panel) recruitment of neutrophils (Ly-6G, magenta) to arterioles 3–5 h post infection with iRFP-expressing Neisseria meningitidis (green). The human vessels are shown in gray (UEA-1 lectin) and dashed lines. Time, hh:min. Scale bar, 30 µm. b Percentage of arterioles recruiting only intraluminal (white circles), only perivascular (red circles), or both intraluminal and perivascular (gray circles) neutrophils. c Numbers of intraluminal and perivascular neutrophils per square millimeter of arteriolar endothelium during the first 6 h of the infection. Data are shown as the mean ± SEM. b, c Quantifications were performed on n = 24 vessels, in total, pooled from N = 7 infected mice imaged independently. d Intravital imaging (maximum intensity z-projection) of neutrophil (Ly-6G, magenta) entrapped within bacterial aggregates within an infected arteriole 7 h post infection with iRFP-expressing Neisseria meningitidis (green). The human vessels are shown in gray (UEA-1 lectin) and dashed lines. Scale bar, 30 µm.
