Fig. 7: The expression of exosome biogenesis/release-associated genes was upregulated in Pkd1 mutant renal epithelial cells and tissues. | Nature Communications

Fig. 7: The expression of exosome biogenesis/release-associated genes was upregulated in Pkd1 mutant renal epithelial cells and tissues.

From: Extracellular vesicles and exosomes generated from cystic renal epithelial cells promote cyst growth in autosomal dominant polycystic kidney disease

Fig. 7

a qRT-PCR analysis of the expression of nSMase2 and Rab27a mRNA in PH2 and PN24 cells (top panel) and in kidneys from WT and Pkd1RC/RC mice (bottom panel). n = 3 independent experiments. All statistical data are represented as mean ± SEM, and p-values are calculated by unpaired Student’s t-test. b Western blot analysis of nSMase2 and Rab27a expression from whole-cell lysates of PH2 cells and PN24 cells (top panel) and in kidneys from WT and Pkd1RC/RC mice (bottom panel). c Immunofluorescence staining indicated that the expression of nSMase2 and Rab27a was increased in cyst lining epithelial cells in kidneys from Pkd1RC/RC mice, which were costained with DBA (Dilichos biflorus agglutinin) but not LTL (Lotus Tetragonolobus lectin). Scale bars, 100 μm. n = 3 independent experiments. d Immunohistochemistry analysis indicated that nSMase2 and Rab27a expression was increased in cyst-lining epithelial cells in human ADPKD kidneys but not that in normal human kidneys. Scale bars, 50 μm. n = 3 independent experiments.

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