Fig. 3: Detection of novel cargo proteins regulated by stress-induced secretory autophagy.
From: Stress-primed secretory autophagy promotes extracellular BDNF maturation by enhancing MMP9 secretion
a CTSD from supernatants measured with ELISA after WT or Atg5 KO SIM-A9 cells were treated with 300 nM dexamethasone (Dex) or vehicle for 4 h. Tukey’s multiple comparison test was performed; ****p < 0.0001. Significance refers to WT Dex compared to every other condition. Data shown as mean ± s.e.m. of n = 3 biologically independent samples. b Volcano plot representation of a multiple t-test analysis of the secretome in which each dot represents the replicates’ mean (difference of WT-Atg5 KO); FDR = 0.01, s0 = 1 of n = 3 biologically independent samples. c Secretion levels of detected cathepsins indicated as fold change of WT over Atg5 KO. Data shown as mean ± s.e.m. of n = 3 biologically independent samples. d GO results performed with Reactome. e GO results performed with SynGO. f Word cloud representation of automated literature mining results in association with “neuroplasticity”.
