Fig. 4: Stress enhances mBDNF production promoting proBDNF cleavage via MMP9.
From: Stress-primed secretory autophagy promotes extracellular BDNF maturation by enhancing MMP9 secretion
a MMP9, b proBDNF, c mBDNF levels from supernatants measured via ELISA after WT or Atg5 KO SIM-A9 cells were treated with 300 nM dexamethasone (Dex) or vehicle for 4 h. d MMP9, e proBDNF, f mBDNF levels from supernatants measured via ELISA after WT SIM-A9 cells were treated with 300 nM Dex, Dex + MMP9 inhibitor I (MMP9i), or vehicle for 4 h. g CTSD, h MMP9, i proBDNF, and j mBDNF levels from supernatants measured with ELISA after WT SIM-A9 cells were transfected with FKBP51 expressing plasmid (ect. FKBP51) or control vector. k CTSD, l MMP9, m proBDNF, n mBDNF levels from supernatants measured via ELISA after WT SIM-A9 cells were treated with vehicle, SAFit, 300 nM dexamethasone (Dex), and 300 nM Dex + SAFit for 4 h. For d, Mann–Whitney, one-tailed test; for g, i, j unpaired, one-tailed t-tests and for h unpaired, one-tailed Welch’s test were performed; for all the others, Tukey’s multiple comparison test was used; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Only significant comparisons are shown. Data shown as mean ± s.e.m. n = 3 biologically independent samples.
