Fig. 5: Stress enhances secretory autophagy and increases the mBDNF/proBDNF ratio in vivo. | Nature Communications

Fig. 5: Stress enhances secretory autophagy and increases the mBDNF/proBDNF ratio in vivo.

From: Stress-primed secretory autophagy promotes extracellular BDNF maturation by enhancing MMP9 secretion

Fig. 5

a Schematic overview of in vivo microdialysis. b Experimental design and timeline; each sample was collected over 30 min indicated by the light gray lines. Quantifications of c CTSD, d MMP9, e proBDNF, and f mBDNF from in vivo mPFC microdialyses of WT and Fkbp5 KO mice. Quantifications of g CTSD, h MMP9, i proBDNF, and j mBDNF from in vivo mPFC microdialyses of WT mice injected intraperetoneally with ULK1 inhibitor (ULK1i) or saline. n = 4 mice per group. Two-way ANOVA with Šídák’s multiple t-test was performed; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Hash symbol refers to time × genotype/treatment interaction. Data shown as mean ± s.e.m. FS foot shock, Fc fold change.

Back to article page