Fig. 2: Cells lacking Gαq/11 display an earlier and prolonged autophagic response upon different types of nutrient stress along with an altered mTORC1 activation status.

a WT and Gαq/11 KO MEFs were maintained in presence or absence of serum during the indicated times (n = 4, except for time 16 h, n = 3). WT (0.1%, 0 h) vs. Gαq/11 KO (0.1%, 0 h), P value = 0.0341. A representative blot of four independent experiments is shown. b WT and Gαq/11 KO MEFs were maintained in the presence or absence of amino acids during the indicated times (n = 5). WT (EBSS, 120 min) vs. Gαq/11 KO (EBSS, 120 min), P value = 0.0407. A representative blot of five independent experiments is shown. For a and b, autophagic markers (LC3-II and p62) and the activation levels of the mTORC1 and AMPK pathways were analyzed by assessing the phosphorylation status of the downstream target of mTORC1 S6 ribosomal protein (see Supplementary Fig. 6a, b for additional readouts of this cascade) or of AMPK, respectively. Tubulin and GAPDH (for the p-AMPK and AMPK blots in panel a) were used as loading controls. Phospho-S6 data (mean ± SEM of the indicated independent experiments) were normalized using total S6 ribosomal protein. Statistical significance was analyzed using two-sided unpaired t-test. For all P values, *P < 0.05. Source data are provided as a Source Data file.