Fig. 1: Structures and the biosynthetic gene cluster of ARTs.
From: Initiating polyketide biosynthesis by on-line methyl esterification
a Structures of ART A-D. The isotope labeling pattern of the ART B aglycone is shown as two separate polyketide chains. The C-1 carbon (marked with a light blue star) was previously proposed to originate from the C-1 of acetate17; in this study, we propose it originates from the free carboxyl end of the malonyl starter unit. b Genetic organization of the art gene cluster. c HPLC profiles of B. subtilis fmb60 wild-type and mutant strains. B. subtilis Δart28/art28 and Δart28/Bc-bioC are two complementation strains of the art28 in-frame deletion mutant B. subtilis Δart28.
