Fig. 3: Characterization of Art9 as an esterase and Art28 as an O-MT. | Nature Communications

Fig. 3: Characterization of Art9 as an esterase and Art28 as an O-MT.

From: Initiating polyketide biosynthesis by on-line methyl esterification

Fig. 3: Characterization of Art9 as an esterase and Art28 as an O-MT.

a The isotope labeling pattern of ART 9B with different 13C-labeled precursors. The C-1 carbon (marked with a light blue star) is proposed to originate from the free carboxyl end of the malonyl starter unit. b HPLC analysis of the enzymatic assays of Art9. c HPLC detection of the methyl esterification of malonyl-ACPArt10 in enzymatic assays of Art28. Deconvoluted mass spectra of apo-ACPArt10, malonyl-ACPArt10, and malonyl-ACPArt10 methyl ester are shown. d LC-MS analysis of SAH production in the enzymatic assays of Art28. EIC extracted ion count.

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