Fig. 7: Enrichment and requirements of coronal synostosis genes for osteogenic cells.
From: The developing mouse coronal suture at single-cell resolution
a Dot plot of genes associated with coronal suture synostosis. The size and color intensity of the dots correspond respectively to the percentage of cells within each cluster expressing the indicated gene, and the average expression level. b Module score for coronal synostosis genes plotted on to the osteogenic/mesenchymal subset UMAP. c–i Combinatorial in situ analysis of coronal sutures for indicated markers at E14.5 or E15.5. Sp7 marks the frontal (F) and parietal (P) bones. c, d Twist1 expression overlaps with markers for PO1 (Cenpf) and OG1 (Erg) within coronal suture mesenchyme of wild-type E15.5 mice. e–i, expression of OG1 markers Erg and Six2 is lost in the suture mesenchyme (arrowheads) of Twist1+/−; Tcf12+/− mice, yet EC1-3 marker Pi16 and LIG marker Tac1 are unaffected. In situs were performed in biological triplicate. Scale bars = 50 µm; images in (c–i) are at the same magnification.
