Fig. 3: ZNF768 depletion affects the expression of key genes controlling proliferation and senescence.

A Schematic overview of the approach designed to test the effect of ZNF768 depletion in various cell lines (HT29, A375, A549, MCF7, HCC155, and Hepg2) using the iLincs resource. B Gene ontology analysis performed with Metascape of the list of genes regulated in at least two cell lines in the analysis described in (A). The full list of gene is presented in Supplementary Data 2. C, D U87 cells were transduced with lentiviruses to knockdown ZNF768. Cells were selected and RNA was isolated 96 h post infection. A microarray was performed from the isolated RNA (n = 3/condition). Gene ontology analysis performed with Metascape on the list of genes identified by microarray that were (C) downregulated or (D) upregulated in response to ZNF768 depletion. The full list of genes is presented in Supplementary Data 4 and 5. E RT-qPCR analysis of gene expression in the samples prepared in (C, D) (n = 3/condition). In all panels, data represent the mean ± SEM. In panel E, significance was determined by two-tailed, unpaired t test. Details about reproducibility are provided in the Statistics and reproducibility included in the Methods section. Source data are provided as a Source data file.