Fig. 6: ZNF768 expression and protein levels are elevated in several human cancers.

A Large-scale cancer genomics analysis of ZNF768 mutation profile performed using cBioportal and TCGA PanCancer Atlas Studies. B Distribution of ZNF768 alteration profile based on the analysis described in (A). C Gene expression analysis of ZNF768 expression between normal and cancer tissues. This analysis was performed using the GEPIA resource. The method for differential analysis is one-way ANOVA, without adjustment for multiple comparisons. The Log2 fold change cut-off was set up at 0.5 and the p-value cut-off at 0.001 (*p < 0.001). Each dot represents one sample. The number (n) of sample analyzed is provided below each graph bar. The low and high limits of the box represent the lower and upper quartiles, respectively. The middle line in the box represents the median value. The whiskers are 1.5 times the interquartile range. D Western blot analyses of ZNF768 protein levels in lung tumors (adenocarcinoma (n = 28) or squamous cell carcinoma (n = 10) or normal lung samples collected from human patients. Representative samples are shown from the same exposure time. E Quantification of ZNF768 protein levels in the experiment described in (D). Significance was determined on log transformed data using two-tailed paired t test. F Immunohistochemistry analyses of ZNF768 protein in normal lung and lung tumors. Examples of tumors showing high levels of ZNF768 are presented (Scale: 100 µm). G Presentation of the proposed functions of ZNF768 in normal and cancer cells. In all panels, data represent the mean ± SEM. Details about reproducibility are provided in the Statistics and reproducibility included in the Methods section. Source data are provided as a Source data file.