Fig. 1: Unsupervised analysis of mononuclear cells in peripheral blood and their characterization.

A Uniform Manifold Approximation and Projection (UMAP) representation of PBMC landscape. Each color indicates a different cell population. CM, central memory; EM, effector memory; TM, transitional memory; CL, classical monocytes; INT, intermediate monocytes; NC, nonclassical monocytes; NK, natural killer cells; γδ, T cells expressing the γδ T-cell receptor; DC, dendritic cells; MAIT, mucosal-associated invariant T cells; LDN, low-density neutrophils; DN, CD4-, CD8- T lymphocytes; DP, CD4+, CD8+ T lymphocytes. B Heatmap representing different clusters identified by FlowSOM, with relative identity and percentages in all groups of subjects. The color in the heatmap represents the median of the arcsinh, 0–1 transformed marker expression calculated over cells from all the samples, varying from blue for lower expression to red for higher expression.