Fig. 2: Synchronization of seta formation.
From: Structural evidence for extracellular silica formation by diatoms
a A synchronized C. tenuissimus culture, where PDMPO fluorescence (green) is observed only in one half of the cell wall. Chlorophyll autofluorescence is in red. Inset shows a bright-field image of the cells. b, c Close-up view of two synchronized cells. Arrows show the newly formed and old valves (green and transparent arrows, respectively). d, e A single cell at the stage of seta formation immediately after the addition of PDMPO. Only the seta tip is labeled with PDMPO (arrows), indicating that this is the forming end of the seta. The rest of the cell is out of focus.
