Fig. 1: Biochemical and cryo-EM studies of LolCDE.
From: Mechanism of LolCDE as a molecular extruder of bacterial triacylated lipoproteins
a Diagram of the steps of lipoprotein biogenesis in the inner membrane, including three enzymatic processing steps, LolCDE-mediated extraction, and delivery to LolA. The Lol proteins are labeled with letters. b ATPase activity of LolCDE in nanodiscs, in DDM, and in nanodiscs with 0.1 mM vanadate. Shown is a representative of three independent experiments. Each point represents mean ± s.d. of three measurements in one experiment. c ATPase activities of LolCDE in nanodiscs. Each point represents mean ± s.d. of three separate measurements. d Cryo-EM map filtered at 3.8-Å resolution and model of LolCDE, viewed from the front (top) and back (bottom). LolC, LolE, two LolD subunits, and lipoprotein are colored separately. Nanodisc is shown as outline. The boundaries of the inner membrane are indicated by gray lines. Hook in LolC, Hook counterpart (HC) in LolE, shoulder loop (SL), and shoulder helix (SH) are indicated. Transmembrane helices are labeled with numbers. e Topology of LolCDE. C C-terminal sequence, CH coupling helix, EH N-terminal elbow helix. f, g Views perpendicular to the membrane plane of the cross sections indicated in (d).
