Fig. 3: Nsun2 ablation alters glycine–serine biosynthesis pathways leading to increased glycine amino acid concentrations in the mutant cortex. | Nature Communications

Fig. 3: Nsun2 ablation alters glycine–serine biosynthesis pathways leading to increased glycine amino acid concentrations in the mutant cortex.

From: Neuronal Nsun2 deficiency produces tRNA epitranscriptomic alterations and proteomic shifts impacting synaptic signaling and behavior

Fig. 3

a Schematic of glycine–serine biosynthesis pathways that are significantly altered in cortex after Nsun2 ablation. Red arrows represent significant FDR adjusted P values <0.1 in unbiased proteomic screen (see Fig. 2b) between Nsun2 KO and WT cortex with average t test differences denoted next to arrows. The green arrow represents significantly changed amino acid concentration in KO vs. WT cortex. b Metabolomic profiling of mutant and WT cortex for 19 amino acids (20 canonical amino acids, except for cysteine; see “Methods”) revealed a significant increase in glycine for Nsun2 KO vs. WT (n = 4/genotype; two-tailed t tests, FDR adj. *P = 0.020; **Grubb’s test for fold changes identified glycine as a significant outlier with alpha = 0.001). Data are presented as mean values +/− SEM. Source data are available as a Source Data file.

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