Fig. 5: Manipulation of neuronal Nsun2 modulates complex behaviors, including cognition and depressive behaviors.

a Behavioral tests of cognition in adult Nsun2 KO and WT mice (n = 12 WT, 7 KO). Left, changes in contextual but not cued fear memory in Nsun2 KO mice compared to WT. Right, no changes in working memory were detected in Y-maze spontaneous alternation (n = 7/genotype; ***P < 0.0001). b Nsun2 KO and WT mice in elevated plus maze (EPM; n = 6 WT M, 7 WT F, 7 KO M, 4 KO F) and light/dark box (L/D; n = 11 WT M, 12 WT F, 10 KO M, 7 KO F; **P = 0.010 for KO vs. WT males post hoc comparisons). c Nsun2 KO vs. WT mice immobility scores in the forced swim (FST; n = 10 WT M, 11 WT F, 10 KO M, 7 KO F) and tail suspension (TST; n = 10 WT M, 11 WT F, 10 KO M, 7 KO F). d AAV8^{hSyn1-Nsun2GFP} vs. AAV8^hSyn1-GFP control mice immobility scores in the FST (n = 10 AAV^GFP M, 10 AAV^GFP F, 6 AAV^Nsun2 M, 10 AAV^Nsun2 F; **P = 0.002 for post hoc comparisons) and TST (n = 10/sex/condition; *P = 0.044 for post hoc comparisons). e AAV8^hSyn1-CreGFP vs. AAV8^hSyn1-GFP control mice immobility scores in the FST and TST (n = 7 AAV^GFP M, 9 AAV^GFP F, 8 AAV^Cre M, 10 AAV^Cre F; ***P < 0^.001). All data are presented as mean values +/− SEM, and all post hoc t tests are two-tailed with Bonferroni correction. Source data are available as a Source Data file.