Fig. 3: Solvent screening for improved conversion at high HMF loading. | Nature Communications

Fig. 3: Solvent screening for improved conversion at high HMF loading.

From: Toward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering

Fig. 3

GOase variants were evaluated for conversion of 150 g/L semi-crude HMF to DFF in the presence of different cosolvents. Conditions: 0.05 g/L pure enzyme, 0.0064 g/L HRP, 880 U/mL Catalase, 100 mM NaPi, 150 g/L Crude HMF in solvent (BASF), 200 µL (80% of aqueous volume) solvent overlay, 20 °C, 24 h. No over-oxidation was observed in any of the samples. DEC, diethyl carbonate. DEM, diethyl malonate. DES, diethyl succinate. EGDA, ethylene glycol diacetate. Each bar represents a single experiment.

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