Table 4 Conversion of HMF to DFF by GOase variant M4 CFE using EtOAc overlay at reduced incubation temperature.

From: Toward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering

Entry

GOase Variant

GOase (g/L)

GOase Form

HRP (g/L)

[HMF]

Type HMF

Conversion (%)

Over-oxidation (%)

1

M4

0.625

CFE

0.0128

250 mM

Pure

96

2.5

2

M4

0.625

CFE

0.0128

25 g/L

Crude

88

6.4

3

M4

0.625

CFE

0.0128

500 mM

Pure

71

0.4

4

M4

0.625

CFE

0.0128

50 g/L

Crude

82

0.8

  1. Data for reactions with purified enzyme provided in Supplementary Table 10. Conditions listed in the table highlight the main differences between samples, while those listed below the table apply to all entries unless otherwise stated.
  2. Conditions: 0.05 mM CuSO4 in GOase M4 CFE reactions, 880 U/mL Catalase, 100 mM NaPi, 200 µL (80% of aqueous volume) EtOAc, 20 °C, 6 h.
Back to article page