Fig. 5: High-differentiation regions maintained by divergent selection at the habitat transition.

The upper row represents the empirically observed difference in AFD (deltaAFD) in the L1-S7 site comparison between high-differentiation regions and randomly chosen regions (n = 178 each). These regions were identified based on the L1-L2 and L1-S1 comparisons. The lower row represents deltaAFD calculated analogously for simulated high-differentiation and control loci evolving to similar baseline differentiation under drift alone (n = 178 each). The black vertical lines indicate median values and gray boxes represent bootstrap 95% compatibility intervals. DeltaAFD observed empirically is much greater than expected under drift alone, indicating that the high-differentiation regions identified in the L1-S1 comparison must be under particularly strong divergent selection between Misty Lake and inlet stream stickleback in general.