Fig. 2: dATPase activity of S-2L PurZ: functional assay and ligand-bound structure. | Nature Communications

Fig. 2: dATPase activity of S-2L PurZ: functional assay and ligand-bound structure.

From: Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA

Fig. 2: dATPase activity of S-2L PurZ: functional assay and ligand-bound structure.

a HPLC profiles representing the time-course of the reaction catalyzed by PurZ in presence of dGMP, Asp and dATP/ATP (purple). Pure compounds used as standards are shown below in black. The enzyme generates products corresponding to (d)ADP and dSMP. b Ribbon representation of a PurZ crystallographic protomer, with dGMP and dATP shown in stick (yellow). c Catalytic pocket of PurZ with the 2Fo − Fc electron density contoured around the reactants at 1 σ (black mesh). Crucial residues surrounding the nucleotides are coloured by chain (purple and orange).

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