Fig. 4: Metabolic pathway of 2-aminoadenine in S-2L phage and result of its introduction in E. coli. | Nature Communications

Fig. 4: Metabolic pathway of 2-aminoadenine in S-2L phage and result of its introduction in E. coli.

From: Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA

Fig. 4: Metabolic pathway of 2-aminoadenine in S-2L phage and result of its introduction in E. coli.

a Native cellular pool of nucleotides is represented in the box to the left; nucleotide pool modified through expression of the viral Z-cluster is shown on the right. Three dots represent unmodified dTTP and dCTP. Structures of S-2L proteins solved in this and previous work13 are shown next to their respective reaction arrows (to scale). Host enzymes (names in grey) finalize the dZTP pathway12. The dotted grey arrow stands for potential use of the standard dNTP pool by PrimPol in the absence of the Z-cluster. b A-to-Z substitution rates in bacterial plasmidic and chromosomal DNA, obtained after complete or partial transplantation of S-2L Z-cluster to E. coli (labels below). Box plot representation highlights data distribution, while black points denote individual expression results (pentaplicates for plasmidic DNA, duplicates for chromosomal DNA). Box whiskers delimit the range of values, box edges mark upper and lower quartiles, vertical lines represent the median, and black crosses—the average. c Accounting for the pre-induction fraction of ATGC-DNA, roughly every fourth plasmidic adenine (green) was changed to 2-aminoadenine (pink) after expressing the Z-cluster.

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