Fig. 2: Elucidation of myo-inositol pathway via 13C-NMR analysis. | Nature Communications

Fig. 2: Elucidation of myo-inositol pathway via 13C-NMR analysis.

From: Conversion of dietary inositol into propionate and acetate by commensal Anaerostipes associates with host health

Fig. 2: Elucidation of myo-inositol pathway via 13C-NMR analysis.

High-resolution 13C-NMR spectra showing [4,513C2]myo-inositol fermentation products that are [3-13C]propionate, [13C]CO2, [1-13C]ethanol, [2-13C]acetate, [1-13C]acetate, [13C]formate (b) with anticipated scheme of 13C flow (c), and [4-13C]myo-inositol fermentation products that are [13C]CO2, [13C]formate, [2-13C]propionate, [2-13C]acetate and [3-13C]propionate (e) with anticipated scheme of 13C flow (f). a and d show concentrations of substrate and end metabolites analysed using high performance liquid chromatography-refractive index detection (HPLC-RI) when the cells were grown in [4,513C2]myo-inositol and [4-13C]myo-inositol respectively. Red arrows indicate the samples time points for 13C-NMR.

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