Fig. 2: Genome-wide screen for CoQ trafficking genes identifies uncharacterized UbiB protein Cqd1.

a Schematic of forward-genetic yeast screen for genes involved in CoQ trafficking. b Growth rates of ∆gpx1/2/3 and four mutant strains resistant to 18:3 treatment (mutA-D). Yeast were assayed in pABA^– media containing 2% (w/v) glucose with 0−25 µM 18:3 (mean ± SD, n = 3 independent samples). c Mutant strains mutA-D were submitted for whole-genome sequencing to identify non-synonymous mutations (total = 442). Mutations were analyzed with PROVEAN to filter for likely deleterious changes (D-score ≤ −4.1, shaded box). Gray, all genes; red, mitochondrial genes. Light, predicted neutral; dark, predicted deleterious. d Growth rate of mutC yeast expressing empty vector (EV) or endogenous CQD1 (mean ± SD, n = 3 independent samples). Yeast were assayed under the conditions described in (b) with 0−100 µM 18:3. e Drop assay of WT, ∆gpx1/2/3, and ∆gpx1/2/3∆cqd1 yeast grown for 3 days on solid pABA^– medium containing 2% (w/v) glucose, 0.5% (w/v) ethanol (EtOH), and 0-25 µM 18:3. A representative drop assay from three independent experiments is shown. f Growth rates of ∆gpx1/2/3 and ∆gpx1/2/3∆cqd1 yeast expressing EV or endogenous CQD1 (mean ± SD, n = 3 independent samples). Yeast were assayed under the conditions described in (b) with 0−50 µM 18:3. Source data for panel (c) is provided as a Source Data file.