Fig. 5: Dpb4 promotes Rad9 association at DSBs independently of γH2A and Dpb11–Rad9 interaction and by acting in the same pathway of Dot1. | Nature Communications

Fig. 5: Dpb4 promotes Rad9 association at DSBs independently of γH2A and Dpb11–Rad9 interaction and by acting in the same pathway of Dot1.

From: Dpb4 promotes resection of DNA double-strand breaks and checkpoint activation by acting in two different protein complexes

Fig. 5

a–d Exponentially growing YEPR cell cultures of JKM139 derivative strains were transferred to YEPRG to induce HO expression. Relative fold enrichment of Rad9-HA (a–c) and Dpb4-HA (d) at the HO-induced DSB at the MAT locus was evaluated after ChIP with anti-HA antibody and qPCR. Strains carrying the hta1-S129A allele also contain the deletion of the HTA2 gene. The mean values of three independent experiments are represented with error bars denoting s.d. ***p < 0.005; *p < 0.05 (unpaired two-tailed Student’s t-test).

Back to article page