Fig. 6: The lack of Dls1 and Isw2, but not of Dpb3, impairs histone removal, MRX association to DSBs, and DSB resection.

a HO expression was induced at time zero by galactose addition to G2-arrested cells that were kept arrested in G2 by nocodazole. Relative fold enrichment of H2A at the HO-induced DSB was evaluated after ChIP with an anti-H2A antibody. The mean values of three independent experiments are represented with error bars denoting s.d. See source data file for statistical analysis. b Exponentially growing YEPR cell cultures were transferred to YEPRG to induce HO expression. Relative fold enrichment of Mre11-Myc at the HO-induced DSB was evaluated after ChIP with anti-Myc antibody and qPCR. The mean values of three independent experiments are represented with error bars denoting s.d. ***p < 0.005 (unpaired two-tailed Student’s t-test). c DSB resection. YEPR exponentially growing cell cultures were transferred to YEPRG at time zero to induce HO production. SspI-digested genomic DNA was analyzed as in Fig. 2a. d Densitometric analysis of the resection products. The mean values of three independent experiments as in (c) are represented with error bars denoting s.d. See source data file for statistical analysis that was performed using unpaired two-tailed Student’s t-test. e Exponentially growing YEPR cell cultures of JKM139 derivative strains were transferred to YEPRG to induce HO expression. Relative fold enrichment of Isw2-HA at the HO-induced DSB was evaluated after ChIP with anti-HA antibody and qPCR. The mean values of three independent experiments are represented with error bars denoting s.d. ***p < 0.005; **p < 0.01; *p < 0.05 (unpaired two-tailed Student’s t-test). f Western blot with anti-HA antibodies of protein extracts from exponentially growing cells. The experiment was performed independently three times with similar results.