Fig. 4: m⁶Am-seq detects m⁶Am methylome at single-base resolution.

a Nucleotide proportion of TSS identified by the sudden drop-off of sequencing reads (left) and by “start rate difference” (SRD) metrics. b A representative view of three single m⁶Am sites on the transcripts of PRR11, which were all supported by CAGE data. Three adenosine residues with a high SRD score (red bar) were defined as m⁶Am sites, which overlapped exactly with CAGE sites (green triangles). Each segment was normalized according to its average length in RefSeq annotation. Pink background color denotes m⁶Am signal. c A metaplot showing that the vast majority of m⁶Am sites by m⁶Am-seq overlapped with CAGE sites, suggesting that these m⁶Am sites are indeed TSSs. Compared with miCLIP, m⁶Am sites identified by m⁶Am-seq were much closer to the CAGE TSSs. Two miCLIP data sets were used for comparison: an m⁶Am list reported in 2015 ²⁰ and an updated m⁶Am list in 2019 ¹⁰, based on PCIF1-KO. Source data are provided as a Source Data file.